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This article is part of the supplement: Proceedings of the First International Cilia in Development and Disease Scientific Conference (2012)

Open Access Poster presentation

The functional relationships between BBS and MKS and their role in regulating trafficking of proteins in the primary cilium

F Bangs*, S Goetz and KV Anderson

Author Affiliations

Sloan Kettering Institute, New York, USA

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Cilia 2012, 1(Suppl 1):P62  doi:10.1186/2046-2530-1-S1-P62

The electronic version of this article is the complete one and can be found online at: http://www.ciliajournal.com/content/1/S1/P62


Published:16 November 2012

© 2012 Bangs et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Poster presentation

Bardet-Biedl syndrome (BBS) and Meckel-Gruber syndrome (MKS) are genetic disorders caused by disruption of primary cilia. The BBS and MKS proteins are found in two distinct protein complexes but the functional relationships between these two complexes are not clear. Here we analyze double mutant mouse embryos to define these relationships. Bbs4;Mks1 double mutant embryos show a stronger phenotype than either single mutant, with completely penetrant micropthalmia and polydactyly, loss of ventral neural cell types, and a reduced number of short bulbous primary cilia. This additive phenotype suggests that BBS and MKS function in parallel pathways. We detected accumulation of Smo and abnormal Gli2 localization in Bbs4;Mks1 double mutant cilia, suggesting that the double mutants have a defect in retrograde trafficking. To define a role of BBS4 or MKS1 in ciliary trafficking, we have generated Bbs4 or Mks1 mice that carry mutations in the IFT machinery. Absence of BBS4 did not affect the phenotype of a weak IFT-B mutant or of embryos that lack the retrograde dynein motor, whereas the Mks1 phenotype was stronger when either IFT-B or dynein were mutant. This implies that BBS works together with IFT to regulate ciliary trafficking whereas MKS1 has a parallel role in ciliary function. Together these data suggest that BBS and MKS both have roles in regulating movement of Hh components in the cilium however the way in which they do this is distinct from each other.