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This article is part of the supplement: Proceedings of the First International Cilia in Development and Disease Scientific Conference (2012)

Open Access Poster presentation

Mechanical strain disrupts primary cilia structure and modulates hedgehog signalling in adult chondrocytes

CL Thompson12*, JP Chapple2 and MM Knight1

Author Affiliations

1 Queen Mary University of London, UK

2 Barts and the London School of Medicine and Dentistry, UK

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Cilia 2012, 1(Suppl 1):P53  doi:10.1186/2046-2530-1-S1-P53


The electronic version of this article is the complete one and can be found online at: http://www.ciliajournal.com/content/1/S1/P53


Published:16 November 2012

© 2012 Thompson et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Poster presentation

Chondrocytes are the unique cellular component of articular cartilage, the connective tissue covering the converging bone surfaces in joints. Chondrocytes within articular cartilage possess primary cilia although their function is unclear. In osteoarthritis hedgehog signalling, which takes place on the cilium, is aberrantly activated promoting cartilage degradation through the upregulation of ADAMTS-5. The mechanisms for this, and the associated increase in cilia length and prevalence, are unknown. This study tests the hypothesis that alterations in mechanical loading influence cilia length and hedgehog signalling leading to increased ADAMTS-5 expression. Mature bovine articular chondrocytes were subjected to 5%, 10% or 20% cyclic tensile strain (CTS). CTS significantly reduced mean primary cilia length (p<0.05) in a dose-dependent manner such that cilia became progressively shorter with increasing strain magnitude. Indian Hedgehog gene expression was significantly increased by CTS at all strains (p<0.05). Pathway activation (Patched1 gene expression), was observed at 5% and 10% strain but not 20% strain where the greatest reductions in cilia length occurred. Similarly, the mRNA levels of ADAMTS-5 were significantly increased by CTS at 5% and 10% strain (p<0.05) but not at 20% strain. These data suggest that mechanical loading activates hedgehog signalling in adult chondrocytes promoting cartilage degradation and highlights a link between primary cilia structure and function in cartilage disease.